New DNA Extraction Method for Long-Read Sequencing in Plant Tissues at the University of Illinois Agilent Case Study: Fragment Analyzer system Novel Genome Assemblies for Plants The University of Illinois Urbana-Champaign researchers Dr. Dessireé Zerpa- Catanho, Dr. Ray Ming, and Dr. Alvaro Hernandez, along with members of their research teams, recently published a unique DNA extraction method. It was designed for the preparation of high-purity, high-molecular-weight (HMW) plant DNA suitable for long-read Oxford-Nanopore next-generation sequencing (NGS). 1 The method will be used to generate long- and ultralong-reads which will then be used for the novel genome assembly of species in the Caricaceae, a family of tropical flowering plants including papaya and other edible fruits.

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