Improving Quantification of Cellular Glycolytic Rate Using Agilent Seahorse XF Technology White Paper Authors Natalia Romero, Pam Swain, Andy Neilson, and Brian P. Dranka Agilent Technologies, Inc. 121 Hartwell Ave. Lexington, MA 02421 Abstract Agilent Seahorse XF Analyzers are used to study cellular metabolism in a diverse array of research areas. Simultaneous measurement of mitochondrial oxygen consumption and glycolytic extracellular acidification rates gives researchers an integrated view of total cellular metabolic activity. Quantification of glycolytic rate is particularly important in immune cell activation and cancer metabolism as these cells dynamically upregulate glycolysis to meet biosynthetic demands. However, the potential for non-glycolytic acidification can

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