azenta.com Consumables and Instruments Cell Freezing Container PBMC Processing CASE STUDYConsumables and Instruments | Case Study Azenta Life Sciences 2 Method trends in PBMC cryopreservation for HIV clinical trials Protocol enhancements over the past 10 years to improve the viability, recovery, and function of PBMCs include media formulation, the use of cold cryoprotectant, the rate of osmotic equilibration during post-thaw washing, the use of pre-chilled sample containers, and pre-cooling the alcohol-filled freezing container (AFC) prior to sample addition. By 2000 the AIDS Clinical Trial Group (ACTG) protocol (1) included working on ice and use of cold cryoprotectant prior to controlled rate freezing to -80oC. While various laboratory SOPs began to specify cold handling after isola

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