azenta.com Storage, Automation & Logistics Best Practices: For Introducing -80°C Bio-Samples Into -190°C Vapor Phase LN2 Freezers WHITE PAPERStorage, Automation & Logistics | White Paper Azenta Life Sciences 2 Abstract Controlled freezing of bio-samples to -80°C followed by placement into a LN2 vapor phase freezer is a common workflow in many labs. Pre-cooled -80°C samples can require several hours to equilibrate to the freezer temperature (circa -190°C). If the samples are exposed as innocents prior to equilibrating they risk thawing above Tg (glass transition). In order to safely keep all samples below Tg (H2O -135°C) users must understand and implement appropriate workflows to ensure that samples are allowed enough time to equilibrate before being exposed during subsequent ro

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