azenta.com Storage, Automation & Logistics Thermal Protection: Protecting Excursions Of Cryogenically Frozen Vials During Transient Warming And Best Practices To Stay Below Tg,H2o WHITE PAPERStorage, Automation & Logistics | White Paper Azenta Life Sciences 2 Introduction Many biological samples contained inside cryoboxes are stored in LN2 vapor-phase freezers at temperatures below -150°C to preserve their viability. The underly- ing assumption is that biological samples show highly reduced degradation and metabolic activity while below Tg (the glass transition temperature). However, every time a cryobox is manipulated or temporarily removed from a LN2 freezer the enclosed samples experience thermal excursions. They could be harmed by inad- vertently crossing the Tg threshold or

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