An Effect of a Shearing Process on the Re-Sequencing of the Arabidopsis thaliana Genome
An Effect of a Shearing Process on the Re-Sequencing of the Arabidopsis thaliana Genome
5 PagesCASE STUDY An Effect of a Shearing Process on the Re-Sequencing of the Arabidopsis thaliana Genome | Case Study 104 | PN400104 1 INTRODUCTION Professor David Guttman is from the Centre for the Analysis of Genome Evolution and Function (CAGEF) at the University of Toronto. His laboratory uses whole genome sequencing and a range of genomic approaches to study the evolution of host specificity and virulence in plant pathogenic bacteria. FRAGMENTATION BIAS During the early stages of Next Generation Sequencing, nebulization was the standard and accepted approach for DNA shearing. According to Pauline Wang (CAGEF Laboratory Facility Manager), their group initially employed nebulization to fragment the Arabidopsis thaliana plant genome for sequencing. Over time, they began to suspe
