Case Study
A comparison of active and passive FFPE DNA extraction methods and effects on downstream analysis metrics
A comparison of active and passive FFPE DNA extraction methods and effects on downstream analysis metrics
summary This case study compares the quantity and quality of dNa extracted using the Covaris truXTra CTM FFPe dNa kit to the QIa GeN® QIa amp® FFPe tissue kit. In the Covaris truXTra C workflow, the focused, short wavelength acoustic field generated by Covaris aF a allows for active paraffin removal without the use of organic solvents, and enables efficient tissue rehydration. This results in a highly simplified workflow, which ensures high yield extraction of nucleic acids. In the QIa GeN QIa amp workflow, paraffin is passively removed by Xylene or other proprietary solvents. FFPe tissue sections from two different human tissue types were used and analyzed using the metrics of: 1. f luorescence microscopy of ffPe sections to determine the extent of paraffin embedding and remo