azenta.com Storage, Automation & Logistics The Effect of Storage Temperature and Repetitive Temperature Cycling: On the Post Thaw Functionality of Human Mesenchymal Stem Cells WHITE PAPERStorage, Automation & Logistics | White Paper Azenta Life Sciences 2 Stem cells are normally transported and stored at either -80°C or below -150°C in LN2 vapor. Best practices typically recommend storing below the glass transition (Tg) of water, approx -135°C. In industry however, there is discussion about, but limited experimental research surrounding cell recovery/viability of -80°C vs. -190°C. Additionally, at either storage temperature samples are typically repeatedly exposed to the ambient environment when adjacent samples are accessed. This temperature cycling is believed to decrease cell viab

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