Monitoring Reverse Phase LC of Acetonitrile/Water in Pharma Applications
Monitoring Reverse Phase LC of Acetonitrile/Water in Pharma Applications
1 PagesPurpose: To provide continuous monitoring of acetonitrile (ACN):water mobile phase gradients utilized in prep scale reverse phase chromatographic separation of active pharmaceutical ingredients with a ClearView db ? ber optic ? lter photometer. Experimental: A spectrophotometer was used to measure the NIR spectra of ACN:water mixtures to select wavelengths for our process photometer. Two meters of low-OH ? ber optic cables were used and t
