Fully Automated High-Throughput Host Cell Protein Analysis of Highly Diverse Samples Background At Novozymes we use bacterial and fungal hosts to manufacture bulk enzymes for a variety of industries. As with all recombinant protein manufacturing processes, host cell proteins (HCPs) are a major source of contamination that can adversely affect product stability and performance. By offering proteome-wide coverage of multiple organisms, mass spectrometry (MS) based HCP analysis is ideally suited to analyze samples from different hosts and strains. However, to keep pace with the large number of samples that our lab is required to analyze, we recognized the need to automate and streamline each stage of our LC-MS approach. Implementing robotic sample preparation, a shortened HPLC separ

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