This Sartorius white paper shows how the iQue Advanced Flow Cytometry Platform accelerates therapeutic antibody discovery by enabling high-throughput, multi-parametric screening. Traditional ELISA and flow cytometry are limited by low throughput, high reagent use, and fragmented analysis. iQue integrates binding, internalization, ADCP, ADCC, and T cell activation assays in one streamlined workflow using small volumes and automated data analysis. Case studies with Her2 and CD20 antibodies demonstrate precise measurement of pharmacological differences, immune responses, and cell viability. The platform’s multiplexing and visualization tools reduce time, cost, and complexity, enabling faster lead identification.

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