Application Note: AN 123B Global Headquarters: 32 Nixon RD, Unit 1, Bolton ON, L7E 1W2, Canada. Email: info@ionics.ca, Phone: 1-905-857-5665, Fax: 1-905-857-3040, www.ionics.ca For Research Use Only Direct Quantitation of 17 ß-estradiol by LC-MS/MS 1. Introduction Many chemicals which are released into the environment have been shown to mimic endogenous hormones such as estradiol 1 . Scientific studies have shown that the

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