CASE STUDY Cell culture supplements Difco TC Yeastolate ultra-filtered (TCY UF) in scale-up optimization Increasing titer to optimize productivity, delivering over 100% improvement Situation A customer asked us to replace a chemically defi ned CHO medium with a higher-performing medium and feed pairing strategy to optimize their results. The goal of this study was to meet or exceed the current baseline titer (control) established in bioreactors. Confi rmation of scalability in bioreactors was also a requirement. Solution A basal medium and feeds were developed after traditional spent media analyses. Following development, a variety of peptones were tested in the basal medium and as feed supplements to improve performance. Process development work was also done to optimize product

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