Continued at the back Application Note 09-2017 Introduction In laboratories, microorganisms such as bacteria, yeast, fungi, animal cells, and plant cells are cultivated in favourable growth environments known as culture media or growth media for various microbiological tests. Culture media contain nutrients, growth promoting factors, energy sources, buffer salts, minerals, metals, and sometimes solidifying or gelling agents (agar) to support the growth and survival of microorganisms. They can be prepared in-house by mixing individual components or purchased commercially as complete dehydrated media and may form as liquid (broth), semi-solid, or solid (agar plate, slant, or deep tube) depending on the solidifying or gelling agent content. Different types of culture med

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