Detecting redside dace on-site using our point-of-need environmental DNA (eDNA) detection platform Background The redside dace (Clinostomus elongatus) is a species of minnow-like fish that is found in small streams in a few isolated watersheds in Ontario. Its appearance is characterized by a half-body length lateral red stripe below a full length yellow streak (Fig 1). Approximately 80% of the Canadian populations are located in the ‘golden horseshoe region’ of southwestern Ontario (Fig 2; MNRF). The species is in serious decline with extirpations recorded in historic locations. As such, the species is listed as Endangered (Species At Risk Act, SARA). The redside dace is especially vulnerable to changes in habitat. Its current distribution is at risk of further dimini

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