Detecting brook trout on-site using our point -of-need environment al DNA (eDNA) det ect ion plat form Background The brook trout (Salvelinus fontinalis) is a common cold- water fish native to Ontario. Brook trout are dark olive- green dorsally, with pale spots and red dots surrounded by blue halos along the flanks. Their fins are red with distinctive white-leading edges (Fig 1). The species is distributed all around Ontario, from small brooks of southern farmlands to the larger rivers, ponds and lakes of the north. However, brook trout live and thrive only in very clean and cold waters. Biologically, they are considered an indicator species due to their exceptional sensitivity to environmental changes, thus revealing the overall health of the ecosystem. Major threat

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