This Thermo Fisher Scientific white paper outlines complete workflows and tools for evaluating genome editing efficiency using CRISPR, TALEN, and other technologies. It details methods from gRNA design to clone validation, supported by Invitrogen and Applied Biosystems platforms. Analysis techniques include GeneArt Genomic Cleavage Detection, Sanger sequencing with SeqScreener software, TOPO cloning, digital PCR, and next-generation sequencing. Each offers trade-offs in precision, throughput, and cost. Case studies show efficiencies ranging from 10–86% depending on method. By combining these tools, labs can optimize genome editing experiments, ensure accurate results, and streamline discovery-to-validation workflows.

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