ISOTEC Case Study No. 80 ENHANCED IN-SITU BIOREMEDIATION (EISBR): DENITRIFCIATION PERMEABLE REACTIVE BARRIER UTILIZING EMULSIFIED VEGETABLE OIL Nitrate Plume Demonstration Test Cape Cod, Massachusetts SITE BACKGROUND ISOTEC was retained to implement a Denitrification Permeable Reactive Barrier (PRB) demonstration test for the treatment of nitrate in groundwater using Emulsified Vegetable Oil (EVO). Septic systems are used to manage nearly 85 percent of the wastewater flow from residences and businesses on Cape Cod. As a result, nitrate emanating from septic systems travels as a plume without significant attenuation in groundwater to coastal waters. The Project represented the first to implement a “Hybrid” approach under the Cape Cod 208 Water Quality Plan, approved by

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